Contaminants From Food Chains Biology Essay

In the monitoring period 2010/2011 this subsystem was exceptionally comprised of five parts, non including the cardinal acquisition of nutrient samples. The system for trying of groceries was entirely modernised to accomplish better effectivity and flexibleness. The first portion of the undertaking deals with monitoring of selected infective bacteriums in the groceries sampled. Isolated bacterial strains are subjected to qualitative survey transcending everyday microbiological analyses. The 2nd portion of the undertaking includes supervising the incidence of toxigenic micromycetes ( molds ) in the sampled groceries. Isolates are identified by genus and species and their toxigenic belongingss are studied ( production of mycotoxins ) . The 3rd portion of the undertaking is devoted to supervising the incidence of genetically modified ( GM ) groceries on the Czech market. This subdivision was included chiefly to fulfill public demand and petitions for informations by the EU and non-governmental organisations, and non because any wellness hazards were expected. The 4th portion of the subsystem is the largest and forms the footing of the whole undertaking, covering with monitoring of dietetic exposure of the population to selected chemical substances. It employs the methodological strategy of the alleged sum diet survey which, unlike regular controls of groceries, includes consumer behavior and a full spectrum of groceries and as such uniquely enables complete word picture of wellness hazards. The 5th subdivision of the undertaking targeted at rating of alimentary consumption was separated from the 4th subdivision in response to the necessity of of all time increasing specialisation of methodological analysiss in the service of nutritionary epidemiology. It includes a separate method of characterizing wellness hazards associated with unequal alimentary consumption.

5.1 System for trying groceries that represent regular population diet in the CR

Collection of nutrient samples was carried out in 32 selected locations in the Czech Republic ( Tab. 5.1.1 ) based on population count ( Tab. 5. 1. 2 ) and divided into 4 parts ( quarter-circles ) . In each location samples are collected in three different stores so as to reflect the comparative size of shopping mercantile establishments in line with consumer penchants. The figure of trying sites depends on financial/capacitive options in order to link with the old sampling system and be representative of parts countrywide. Over the biennial monitoring rhythm samples were collected from 96 mercantile establishments across 32 countries in the CR and over 8 periods of clip so as to cover expected seasonal alterations in nutrient supply.

Tab. 5.1.1 Sampling vicinities in the market web 2010/2011

Term I

19.1-2.3 2010

18.1.-1.3. 2011

Term II

23.3.-2.5. 2010

22.3.-3.5. 2011

Term III

25.5.-14.9. 2010


Term IV

5.10.-23.11. 2010

4.10.-22.11. 2011


Kutna Hora ProstA›jov

Blansko PelhA™imov








A?eske BudA›jovice





Mlada Boleslav






NamA›A?A? n. Osl.



ValaA?ske MeziA™iA?i





Uherske HradiA?tA›

Tab. 5.1.2 Choice of shopping vicinities and no. of purchases harmonizing to size of municipality ( EHIS CR, 2009 )


% population

No. of mercantile establishments

No. of purchases

over 100A 000 dad.




50A 000 – 99A 999 dad.




20A 000 – 49A 999 dad.




10A 000 – 19A 999 dad.




5A 000 – 9A 999 dad.




2A 000 – 4A 999 dad.




to 1 999 dad.








5.2 Bacteriological analysis of groceries

The survey focused on bacteriological analysis of groceries involved supervising the happening of selected pathogens in nutrients available in the market web. Choice of trade goods was based on the nutrient basket and, as in old old ages, targeted at those nutrient groups that had in the past participated in the happening of alimental diseases in the Czech Republic or abroad.

Foods were examined for the presence of four aetiologic agents doing important alimental diseases: Salmonella spp. , Campylobacter spp. , Listeria monocytogenes and S. aureus. Except for salmonella and L. monocytogenes the other agents are monitored merely exceptionally during everyday nutrient safety reviews. Information sing their incidence in several trade goods and elaborate phenotype and genotype features are unavailable.

Detection and finding of pathogen counts were carried out utilizing mention cultivation methods ( EN ISO ) . Suspected settlements were confirmed and characterised in item by pheno- and genotyping. For sensing of Salmonella spp. , a sum of 276 nutrient samples was examined. Samples comprised assorted types of meat, dairy and fish green goods, eggs, dumplings, fruit and veggies. None of the samples were positive for presence of the mark pathogen. For sensing of Campylobacter spp. , a sum of 156 nutrient samples was examined. Samples comprised meat, dairy merchandises ( frozen sweets ) , fruit and veggies. None of the samples were positive for presence of the mark pathogen. For sensing of Listeria monocytogenes, a sum of 264 nutrient samples were examined, giving 18 ( 6.5 % ) isolates. L. monocytogenes was detected in land meat ( 5/41.7 % ) , beef ( 8/33.3 % ) and fish ( 3/12.5 % ) . In one instance the pathogen was present in smoke-cured meat ( 1/4.2 % ) and in a smear taken from apple rinds ( 1/4.2 % ) . For sensing of Staphylococcus aureus, a sum of 276 nutrient samples was examined, giving 22 ( 8.0 % ) isolates. All examined nutrient samples contained counts of coagulase-positive staphylococcus & lt ; 50 KTJ/g with the exclusion of one sample of dumplings with 9.102 KTJ/g. Positive findings of the monitored pathogens in peculiar nutrient trade goods are shown in Fig. 5.1.

5.3 Mycological analysis of groceries

In 2011, the 2nd twelvemonth of a biennial monitoring period ( 2010-2011 ) , it was continued as portion of the MYKOMON survey to supervise the incidence of toxicogenic filiform microscopic Fungi ( molds ) , manufacturers of aflatoxins and ochratoxin A in selected groceries. Specialized mycological scrutinies continued to be focused on the above issues, with more elaborate mycological monitoring of microscopic Fungis Aspergillus belonging to the Nigri group, manufacturers of ochratoxin A.

A sum of nine types of consumer nutrients, consisting 108 nutrient samples, was collected from 12 trying sites countrywide on two occasions. Frequency information was collected refering qualitative and quantitative incidence of toxicogenic filiform microscopic Fungi, manufacturers of aflatoxins and ochratoxin A, in nutrients in the Czech Republic. Entire counts of fungous content in selected nutrients ( KTJ/g ) were determined along with their mycological profiles and taint indices ( Ik ) which represent the ratio of potentially toxicogenic filiform microscopic Fungi to the overall count filiform microscopic Fungi ( KTJ/g of groceries ) .

The presence of aflatoxin-producing Aspergillus flavus was detected in 11 samples ( 18 % ) of cereal babe pudding, fruit tea, black tea, assorted types of flour. The presence of ochratoxin A-producing Aspergillus from the Nigri group was detected in 30 samples ( 63 % ) of raisins, fruit tea, black tea and cereal babe pudding. The presence of potentially toxicogenic filiform microscopic fungi Penicillium crustosum ( possible manufacturers of the mycotoxin penitrem A ) was non detected in walnuts during this monitoring period.

5.4 Incidence of GMO nutrients in the Czech market web

Monitoring of selected nutrients on the market to place merchandises that are genetically modified or derived from genetically modified being has continued for the 10th twelvemonth running. As in old old ages, samples of four types of nutrient ( soya beans, soybean merchandises, cornstarch and rice ) were collected from 12 distribution sites countrywide, on four occasions. A sum of 192 samples ( 48 from each trade good ) were collected. Screening and polymerase concatenation reaction ( PCR ) were used for sensing and designation of GM nutrients.

In 2011, qualitative PCR revealed 31 samples of cornstarch, 14 samples of rice and 1 sample of soya beans as GM positive. PCR applied to samples of cornstarch identified the presence of genetically modified maize line MON810 ( 1 sample ) , NK603 ( 3 samples ) and NK603 x MON810 ( 5 samples ) . Familial alteration failed to be detected in 22 samples of maize flour. All positive samples were tested for Bt63 alteration, with negative consequences. The specific type of alteration nowadays remains undetected. One sample of soya beans contained GM Roundup Ready soybean.

Consequences show ( Tab. 5.4.1 ) that GM nutrients or nutrients incorporating GM maize, rice and soya beans are normally found on in the market web of the Czech Republic. The Numberss of positive findings in the monitored nutrient sorts during the period 2002 – 2011 are shown in Fig. 5.2. During 2011 no new scientific information has been published associating to possible wellness hazards ensuing from ingestion of GM nutrients.

Tab. 5.4.1 Consequences of nutrient sample proving for GMO content, 2011


Sample size

Positive findings ( % )

Negative findings ( % )

Soya beans


1 ( 2,1 )

47 ( 97,9 )

Soy merchandises


0 ( 0,0 )

48 ( 100,0 )



14 ( 29,2 )

34 ( 70,8 )



31 ( 64,6 )

17 ( 35,4 )



46 ( 24,0 )

146 ( 76,0 )

5.5 Dietary exposure

The purpose of this long-run monitoring programme is a point appraisal of both mean and specific population group exposure to selected chemicals ( contaminations, nutrients/micro-nutrients ) in the Czech Republic ; this appraisal has been followed up as a chronic exposure tendency over a longer period. The information obtained assist the word picture of wellness hazards associated with the usual dietetic wonts of the Czech population and, if necessary, the chance appraisal of chronic exposure doses. Such appraisal is carried out in 4 – 6 twelvemonth intervals, upon accumulating a sufficient volume of informations. The chemical content of nutrients may stand for hazard of oncological or other diseases. In the instance of foods and micro-nutrients the hazard of deficient consumption is similarly an issue.

Collected nutrient samples are gathered at a individual installation in the Czech Republic where they are subjected to standard culinary intervention and later analysed for content of selected chemical substances. Since 2004 the monitoring of dietetic exposure has been performed in biennial intervals. The sampling system is sufficiently representative for the existent diet of the CR population ( selected nutrient types represent over 95 % of the mass of diet composing ) but non for comparing of regional differences ; such a sampling system is limited by available fiscal resources.

Appraisal of exposure doses during the 2010 – 2011 monitoring period involved usage of two values of expected nutrient ingestion: ‘actual value of ingestion ‘ ( from the national epidemiological survey of single nutrient ingestion , which presents values of average nutrient consumption per individual in the CR for 2003/2004 ) and for rating of exposure trends a value derived from the theoretical account of recommended nutrient doses ( alleged nutrient pyramid ) .

5.5.1 Choice of samples for analysis

The set of samples collected for chemical analysis comprised 205 separate nutrient types from 32 locations in the CR ( see debut of chapter ) . The overall figure of samples ( some types were collected repeatedly and from more than one trade name ) was 3 696 nationally over a period of 2 old ages. For economic grounds the nutrient samples are combined into alleged composite samples harmonizing to part ( quadrant ) . Samples representative of each part were subjected to standard culinary intervention and classified into 143 single types of composite samples for each of the four parts of the Czech Republic. Some samples/composites are prepared repeatedly so that the entire figure per part is 220 composite samples over the whole monitoring period. A sum of 880 composite samples was available for analysis of chemical content over the monitoring period and from the whole of the CR. Certain analytical finding ( e.g. nitrites and nitrates ) employ different and specific choice or combinations of nutrient samples.

5.5.2 Chemical analyses and calculation of exposure doses

A sum of 93 single chemical substances were quantified in the nutrient samples, frequently organizing groups of related substances with similar wellness effects ( for case, 37 types of fatty acids ) . Detected concentrations of chemical substances were used to gauge average exposure doses for the CR population in 2010/2011. For long-run comparing of exposure doses since 1994, a theoretical account of recommended nutrient doses for the CR was used. This is calculated for 5 typical population groups ( kids, work forces, adult females, pregnant/lactating adult females, and the aged ) and allows standardisation of consequences to let long-run monitoring of tendencies in concentration alterations of chemicals in groceries, independently of fluctuations in nutrient ingestion.

5.5.3 Exposure to organic contaminations

Mean chronic population exposure doses to supervise organic substances belonging to the group of alleged relentless organic pollutants proscribed by the Stockholm Convention ( polychlorinated biphenyls , aldrin, endrin, dieldrin, methoxychlor, endosulfan, heptachlor epoxide, hexachlorobenzene , alpha- , beta- , delta- , gamma- ( lindane ) isomer of hexachlorocyclohexane, isomers of DDT, DDD, DDE, alpha- , gamma- , oxy- chlordane, mirex ) from nutrients did non in 2010/2011 exceed values associated with significantly increased chance of wellness harm ( non-carcinogenic ) . Exposure as estimated by existent ingestion of groceries ( SISPO4 ) was highest for PCB. Exposure to the amount of seven index congeners of PCB ( alleged NDL-PCB ) reached a average value of 2.1 % of the tolerable day-to-day consumption ( CZ-TDI ) . This value is somewhat lower than in old old ages.

A high figure of analytical gaining controls has ever been observed for DDT metabolite P, p’DDE ( 56 % ) . Increased Numberss of gaining controls were besides recorded for hexachlorobenzene ( 47 % ) , delta HCH ( 39 % ) and P, P, ‘DDT ( 37 % ) .The fluctuating sum of gaining controls over the old ages is associated with low measured concentration values and attendant low exposure doses ( for case, 0.1 % of PTDI for the amount of DDT and 0.9 % of TDI for hexachlorobenzene ) . Results confirm a prevailing cover taint by these relentless organic pollutants, albeit in really low concentrations that do non show important wellness hazard when they are evaluated as single chemical substances.

Estimates were non made in 2010/2011 of exposure doses for substances with alleged dioxin consequence ( toxic equivalent of 2,3,7,8-tetrachlorodibenzodioxin for the amount of 29 dioxin-like congeners of PCB, dioxins and dibenzofurans ) because of budget cuts.

Exposure doses estimated utilizing the recommended nutrient doses theoretical account have highest values for the 4 – 6 old ages age-group. Exposure to the amount of seven index congeners of NDL-PCB was 8.4 % of TDI amongst these kids. Exposure doses of polychlorinated biphenyls are lower than in the yesteryear ( Fig. 5.3 ) .

5.5.4 Exposure to inorganic substances and ions

This subdivision is focussed entirely on measuring toxic doses of inorganic substances and ions and is non concerned with the issue of nutritionary adequateness of mineral consumption. The average chronic population exposure dose based on existent nutrient ingestion ( SISPO4 ) for nitrates, nitrites cadmium, lead, arsenic, Cu, Zn, manganese, Se, chrome, Ni, aluminum, Fe, I, Sn and molybden did non transcend exposure bounds for non-carcinogenic effects. Exposure to nitrates and nitrites was 23 % and 25 % ADI, severally. Mean manganese consumption was 35 % of the mention dosage ( RfD ) . Cadmium burden was 49 % of the tolerable hebdomadal consumption TWI ( EU ) . Although lead burden remained at practically the same degree as in the old period, the methodological analysis of toxicological rating has changed and has stricter parametric quantities. Detected lead exposure for the mean population is 0.18 Aµg/kg organic structure weight/day. In footings of cardiovascular toxicity comparing with BMDL01outputs MOE= 8.3 which is considered acceptable. For lead nephrotoxicity MOE = 3.5 which is besides merely acceptable. In footings of developmental neurotoxicity in kids, the theoretical account for kids aged 4 – 6 old ages shows 0.65 Aµg/kg organic structure weight/day which outputs MOE = 0.77. Negative effects can non be ruled out and it is presently non possible to gauge the figure of affected kids. Exposure to methylmercury from fish and seafood was merely 2.1 % PTWI and exposure to entire quicksilver from other nutrients was about 0.8 % PTWI. Intake of Cu and Zn continues to be at toxicologically low degrees of 2.8 % and 15 % PMTDI, severally. Estimated population exposure to alleged ‘toxic arsenous anhydride ‘ ( estimation of the amount of inorganic As compounds ) reached 0.08 Aµg/kg organic structure weight/day, which compared to the lowest BMDL01 outputs MOE = 4.3 – 8.6 for lung malignant neoplastic disease. This consequence is notable, albeit with uncertainness. Selenium reached a value of 14 % RfD as in the old period. Estimates of exposure doses to nickel ( 7 % ) and chrome ( 21 % ) fluctuate somewhat year-on twelvemonth. Estimates of population aluminum exposure were 21 % PTWI ( in kids aged 4 – 6 old ages values exceeded PTWI ) and 16 % PMTDI for Fe ; these figures do non stand for a menace to consumer wellness safety. Mean iodine consumption was 13 % PMTDI. Tin was detected in merely 8 relevant nutrient types ( canned meat, canned pate, canned fish, sterilised veggies, vegetable purees, canned fruit, jams, marmalades, fruit-based babe nutrient ) with exposure of merely 0.04 % PTWI. Estimated Mo exposure was 37 % RfD.

The exposure dosage estimated harmonizing to theoretical accounts of recommended nutrient doses by and large reached highest values in the 4 – 6 old ages age-group. Estimated exposure to nitrates was about 101 % ADI ( including burden from veggies ) ; exposure to entire Mn was 147 % RfD. The wellness impact of this consequence is hard to construe because the chemical signifier of Mn was non determined, but may be preliminarily described as serious. Of go oning involvement is the development of Se exposure harmonizing to recommended dosage theoretical accounts ( Fig. 5.4 ) because its consumption is somewhat but invariably on the addition in population groups.

5.6 Evaluation of alimentary consumption

In the 2010/2011 period it was decided to concentrate this undertaking on the methodological analysis of measuring alimentary consumption in kids because this is the most discussed issue in all nutrition surveies. National Institute of Public Health joined the international EFSA undertaking PANCAKE ( Pilot survey for the Assessment of Nutrient consumption and nutrient Consumption Among Kids in Europe ) aimed at the creative activity of consonant methodological analysis for roll uping informations on kid nutrient ingestion in Europe up to 2020. Part of this undertaking were pilot surveies carried out in the CR and Belgium, with the aid of the Netherlands and Denmark, which verified the viability of proposed processs and the quality of undertaking necessities. In the CR the pilot survey involved a sum of 231 topics who were indiscriminately selected from the cardinal registry of occupants for Brno-City and Brno-Rural parts, harmonizing to specific standards ( gender, age, topographic point of abode ) . The research targeted the undermentioned age-groups: babies ( 3 – 11 months ) , yearlings ( 12 – 35 months ) , kids ( 3 – 10 old ages ) every bit good as nursing female parents who participated with their babies. Determination of nutrient ingestion took topographic point from January 2011 – July 2011 by manner of a three-day record and twice repeated 24h callback combined with a record. New EPIC-Soft package developed by the International Agency for Research into Cancer ( IARC ) was used for informations aggregation. Collected informations were farther processed including a basic nutritionary appraisal aimed peculiarly at finding the distribution of usual nutrition consumption in the monitored population groups. During this period the consequences for energy, proteins, fats, carbohydrates, Ca, phosphoric and vitamin C were processed.

The ensuing informations can non be considered representative for the CR in position of the little figure of respondents. However, they provide alone methodological and epidemiological information on nutrition consumption in little kids which is non presently available in the CR in comparable quality. Consequences are hard to construe for the babies age-group because 64 % of the topics were to the full or partly breast-fed and nutritionary consumption via human milk was non monitored. Data for this age-group are hence non presented. During rating of usual consumption in the kid age-group the wide age-range of this age group, 3 – 10 old ages, should be borne in head.


The average value of regular energy consumption in the babies age-group was 4 725 kJ/d, in the kid group 6 837 kJ/d and in the lactating female parents group 8 991 kJ/d. These values correspond to energy demands for the given age-groups. Comparison was carried out with EU ( 1993 ) and DACH ( 2002 ) recommendations.

Proteins, fats and carbohydrates

Nutrient consumption was expressed as a ratio of entire energy consumption. Proteins, fats and carbohydrates were at ratios of 14 % : 32 % : 54 % in babies and kids, and 15 % : 37 % : 48 % in the nursing female parents. All values correlated with recommended values although fat consumption was somewhat higher in the nursing female parents. For carbohydrates a higher consumption of added sugars against WHO ( 2003 ) recommended value ( & lt ; 10 % ) was recorded in all age-groups: 14 % in babies, 17 % in kids and 14 % in nursing female parents. Besides consumption of concentrated fatty acids ( SFA ) was higher than the WHO ( 2010 ) recommended values ( & lt ; 8 % in yearlings and kids, & lt ; 10 % in nursing female parents ) in all age groups: 14 % in babies, 13 % in kids, 15 % in nursing female parents.


The average value of regular Ca consumption was 774 mg/d in the babies age-group, 768 mg/d in the kid age-group and 927 mg/d in the nursing female parents. Comparison with available recommendations ( Ear, USA ) shows that estimated consumption is unequal in 25 % of the babies and nursing female parents groups and about 50 % in the kid age-group ( Fig. 5.5 ) .


The average value for usual phosphoric consumption in the babies age-group was 864 mg/d, in the kid age-group 1 132 mg/d and in the lactating female parents 1 559 mg/d. Intake is considered equal in all monitored age-groups when compared to recommendation ( EAR, USA ) . Current detected values pose no menace from inordinate consumption, as caused by nutrient additives, which is widely discussed in the instance of phosphoric. Estimated population exposure doses are 19 mg/kg organic structure weight/d ( 1 216 mg/person/d ) , which harmonizing to toxicological parametric quantities is 41 % of the doses 3 000 mg/person/d determined by EFSA ( 2006 ) as the alleged UL.

Vitamin C

The average value of usual vitamin C consumption in the babies group was 55 mg/d, in the kid group 50 mg/d and in the nursing female parents 55 mg/d. Comparison with available recommendations ( EAR, USA, Fig. 5.6 ) shows that up to 90 % of nursing female parents had deficient vitamin C consumption. Intake in the babies and kid groups appears equal ( inadequacy prevalence & lt ; 5 % ) . In the instance of the kid age-group the rating is debatable due to the greater age-range against recommended values.

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